Generation of stable mouse neuroblastoma N2a cells that express either wild-type human APP695 N2a APP695wt or human APP695swe N2a APP695swe

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INVENTION NOVELTY: Researchers have generated transgenic mouse N2a neuroblastoma cells that stably express wild-type or mutated APP, respectively. C-terminally myc-tagged wild-type APP, or APP carrying the Swedish familial Alzheimer's disease (FAD)-specific amino acid substitution were cloned in plasmid pCB6 under control of CMV promoter. Next, mouse N2a neuroblastoma cells were transfected with either construct and stabilized using antibiotic treatment. Presence of recombinant protein was validated via anti-myc immunoblotting and immunoprecipitation.

The stable cell lines will allow researchers to examine the intracellular processing of wild-type or mutated APP and the production of Ab peptides, the principal component of amyloid plaques in Alzheimer's disease.

ASSOCIATED PUBLICATIONS: J Biol Chem. 271(16):9390-7.
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Christine Joseph
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