C13683 - Anti-Tmem100 Antibody

Case ID:
C13683
Disclosure Date:
6/3/2015
Description:
Invention Novelty:
The Johns Hopkins researchers have developed rabbit polyclonal antibody specifically against Tmem100 that is suitable is suitable for immunohistochemical staining of tissue sections and western blot to detect Tmem100 protein level.
 
Value Proposition:
Tmem100 is a 134-amino acid, two-transmembrane protein highly conserved in vertebrates (Moon et al., 2010) and is identified as a candidate for the modulation of the TRPA1-V1 complex in the nociceptive pathway in the dorsal root ganglion (DRG) (Weng et al., 2015). Tmem100 has been shown to be involved in processes such as renal development (Georgas et al., 2009), vasculogenesis (Moon et al., 2010), and lung cancer cell invasiveness (Frullanti et al., 2012). Therefore, it is important to have a specific antibody to detect Tmem100 protein level. The JHU researchers have established a rabbit polyclonal antibody specifically against Tmem100 that is suitable is suitable for immunohistochemical staining of tissue sections and western blot to detect Tmem100 protein level. This will provide researchers with important information on the protein function in various biological processes and diseases.
 
Technical Details:
The JHU researchers have identified Tmem100 as a candidate for the modulation of the TRPA1-V1 complex in the nociceptive pathway in the dorsal root ganglion (DRG) (Weng et al., 2015). Tmem100 is a 134-amino acid, two-transmembrane protein highly conserved in vertebrates (Moon et al., 2010). It is found in other organs besides the DRG, expressed in blood vessels, ventral neural tubes, and the notochord (Moon et al., 2010). Tmem100 has been shown to be involved in processes such as renal development (Georgas et al., 2009), vasculogenesis (Moon et al., 2010), and lung cancer cell invasiveness (Frullanti et al., 2012). Therefore, it is important to have specific antibody to detect Tmem100 protein level. The JHU researchers have generated rabbit polyclonal antibody against Tmem100. They demonstrated that this antibody is suitable for immunohistochemical staining of tissue sections and western blot to detect Tmem100 protein level (Weng et al., 2015). The researchers found that Tmem100 staining in the DRG from Tmem100 knockout mice using the antibody was completely abolished indicating the antibody is highly specific Tmem100 protein. Furthermore, the expression of Tmem100 is significantly increased in the DRG from wild-type mice with inflammation supporting our finding that Tmem100 plays essential role in inflammatory pain by regulating TRP channels. Therefore, the expression study of Tmem100 by using the antibody will provide the researchers with important information on the protein function in various biological processes and diseases.
 
Publication(s)/Associated Cases:
Neuron. 2015 Feb 18;85(4):833-46.
 
 
Patent Information:
Inventors:
Category(s):
For Information, Contact:
Nakisha Holder
nickki@jhu.edu
410-614-0300
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