R-3327-AT6.3

Unmet Need
There is increasing evidence supporting that ‘loss of function’ of metastasis suppressor genes plays an important role in cancer metastases. To help identify metastasis suppressor genes on human chromosomes, Johns Hopkins researchers have transferred individual human chromosomes into metastatic rat prostate cancer cells.
 
Technology Overview
To identify metastasis suppressor genes on human chromosomes, Johns Hopkins researchers transferred individual human chromosomes (7, 8, 10, 11, 12 or 17) into highly metastatic Dunning R-3327 rat prostate cancer cells by microcell-mediated chromosome transfer. In these studies, introduction of human chromosomes into the highly metastatic rat prostate cancer cells resulted in suppression of the metastatic ability without suppression of the tumorigenicity. The resultant microcell hybrids were analyzed to determine which portion(s) of human chromosome suppressed the metastatic ability of the rat prostate cancer cells.
 
Stage of Development
Hopkins inventors have created a series of highly metastatic prostate cancer cell line which grow both in vitro and in vivo.
 
Publications
Nihei N, et al. Genes Chromosomes Cancer. 1995 Oct;14(2):112-9.
Nihei N, et al. Cancer Res. 2002 Jan 15;62(2):367-70.
Ichikawa T, et al. Asian J Androl. 2000 Sep;2(3):167-71.
 

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