miR-143/145 conditional knockout mice

Unmet Need
The miR-143/145 microRNA cluster has been implicated in colon cancer and other epithelial tumors, but investigation of its role in pathogenesis requires a research tool in which miR-143/145 expression can be modulated in specific cell types.
 
Technology Overview
Johns Hopkins researchers generated a conditional knockout allele for the miR-143/145 cluster that has been used to develop transgenic mice. miR-143/145 conditional knockout mice have been generated, leading to an initial validating study on the effect of miR-143/145 deletion specifically in epithelial cells of the gastrointestinal tract.
 
Stage of Development
Both miR-143/145^Null/+ and conditional miR-143/145^flox/+ strains have been transferred to the Jackson Laboratory for dissemination. Jackson laboratories have designated the relevant mouse strains as:
028424 B6.129-Mirc32tm1Jtm/J
028503 B6.129(Cg)-Mirc32tm1.1Jtm/J
028504 B6.129(Cg)-Mirc32tm1.2Jtm/J
 
Publications
Cell. 2014 May 22;157(5):1104-16.

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