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ABSTRACTJHU researchers generated mice carrying a targeted deletion of recently characterized Pac gene. CRISPR/Cas9-mediated homologous recombination was performed to generate Pac floxed allele in mice. ES cell clones with proper targeting were identified by PCR and sequencing analysis. Two correctly targeted ES clones were chosen to generate chimeric...

Published: 6/28/2024 | Inventor(s): Zhaozhu Qiu

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Category(s): Clinical and Disease Specializations > Neurology > Cerebral Ischemia, Technology Classifications > Research Tools > Animal Models