Manipulating carbohydrate processing pathways in insect and other eukaryotic cells so that the cells produce complex sialylated glycoproteins is useful for enhancing the value of heterologous expression systems and increasing the application of heterologous cell expression products as vaccines, therapeutics, and diagnostic tools.
Technical Details:
This technology is compositions and methods for producing glycoproteins that have sialylated oligosaccharides. Particular, it is directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrates engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes.
Looking for Partners:
The methods and compositions are useful for heterologous expression of glycoproteins.
