A Simplified System for Generating Recombinant Adenoviruses

Case ID:

C03356

C03356: A Simplified System for Generating Recombinant Adenoviruses
Technical Details:
Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. A strategy, which simplifies the generation and production of such viruses is reported here. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulation, employing homologous recombination in bacteria rather than in eukaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein. Homogeneous viruses can be obtained form this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes.

Direct Link:

https://jhu.technologypublisher.com/technology/16135

Inventors:

Category(s):

Clinical and Disease Specializations, Clinical and Disease Specializations > Infectious Diseases, Technology Classifications > Research Tools > Viruses, Technology Classifications > Therapeutic Modalities, Technology Classifications > Research Tools,

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For Information, Contact:

Jeanine Pennington

jpennin5@jhmi.edu

410-614-0300

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