C05076: Histone Mutant Plasmid Collections Technical Details:
Epigenetics, the study of stable alterations in gene expression that arise during development and cell proliferation, is a popular area of modern bioscience. The epigenetic context in which genes are expressed is important for understanding normal phenotypic variation and disease. DNA methylation has been the most comprehensively studied epigenetic mark to date, but numerous studies involving histone modification and chromatin structure are now being conducted. Recently, scientists at the Johns Hopkins University created a novel mutant histone plasmid collection that will aid in the understanding of chromatin as a key modifier of gene expression, recombination and DNA repair.
Publications/Associated Cases:
1. Hyland, E., Cosgrove, M.S., Molina, H., Wang, D., Pandey, A. Cotter, R., Boeke, J.D. (2005) Insights into the Role of Histone H3 and Histone H4 Core Modifiable Residues in Saccharomyces cerevisiae. Mol. Cell Biol. 25: 10060-70
2. Park, J., Cosgrove, M., Youngman, E., Wolberger, C., and Boeke, J. D. (2002). A core nucleosome surface critical for transcriptional silencing. Nature Genet. 32,273-279.
