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PET32a O-GlcNAcase-thioredoxin Expression Vector
Case ID:
C10105
Report of Invention:
6/22/2007
Web Published:
10/7/2014
C10105: PET32a O-GlcNAcase-thioredoxin Expression Vector
Technical Details:
Expression and Purification of Recombinant and Endgenous 0- GlcNAcase The human O-GlcNAcase cDNA was subcloned into the commercial expression bacterial expression vector PET32a (Novagen, Madison, WI), as was the shorter splice variant (MGEA5 s), to yield fusion proteins with thioredoxi. Both construct were confirmed by sequencing.
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This expression vector is used to express active O-GIcNAcase in E. coli. It produces a fusion protein between thioredoxi and O-GIcNAcase which allows for active O-GIcNAcase to be produced in E. coli.
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Direct Link:
https://jhu.technologypublisher.com/technology/16591
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Category(s):
Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools,
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For Information, Contact:
Christine Joseph
cjoseph6@jhmi.edu
410-614-0300
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