C10183: Fibroblast Cell Line with Cloned Mammalian Na+/H+ ExchangersNovelty:
A fibroblast cell line stably transfected by three isoforms of the Na+/H+ exchanger, NHE-1, NHE-2 and NHE-3, to assess Na+/H+ exchange function with null background.
Value Proposition:
Na+/H+ exchangers, NHEs, maintain the electrochemical gradient in mammalian cells by catalyzing the exchange of extracellular Na+ for intracellular H+. This invention is a tangible material whereby fibroblast cell line is stably transfected by NHE-1, NHE-2 and NHE-3. These cells are ideal for the Na+/H+ exchanger functional studies as well as drug effect studies with null background. Additional advantages include:
• NHE-1 aids in the development of treatment for cardiac ischemia
• NHE-2 can be used to study Na+ absorption and function for treatment of diarrhea when NHE-3 is inhibited by cAMP/cholera toxin
• NHE-3 is essential in studies related to diarrhea and constipation
Technical Details:
Johns Hopkins researchers have cloned, sequenced and expressed NHE-1, NHE-2 and NHE-3 in PS120 cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNAs were cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then stably transfected using double selection with G418 and acid selection. The Na+/H+ exchange activity of these cells were proven using fluorescence measurement using a pH sensitive dye.
Looking for Partners:
To develop & commercialize the technology as a research tool to assess Na/H exchanger function and drug response.
Stage of Development:
Cell line established
Data Availability:
Data validated
Publications/Associated Cases:
J Biol Chem. 1993 Jun 5;268(16):11917-24
