C10434: Reagents for gene methylation assays. Technical Details:
Plasmid DNAs incorporating human gene-specific promoter sequences altered by sodium bisulfate treatment. The plasmids contain sequences hybridizing to external QM-MSP regions within gene promoters of RASSF1A, TWIST1, NIN1, and HIC1. These areas bracket cloned DNA from non-human sources to serve as reference control for novel methylation approaches, including studies of blood. Each region cloned into an individual plasmid.
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Their use includes serving as reagents, standards, or controls for assays of gene methylation (e.g. for determining methylated or methylated gene copy number or for calibration of assay parameters or equipment).
Publications/Associated Cases:
1. Fackler MJ, Malone K, Zhang Z, et al. Quantitative multiplex methylation-specific PCR analysis doubles detection of tumor cells in breast ductal fluid. Clin Cancer Res 2006; 12:3306–10.
2. Fackler MJ, McVeigh M, Mehrotra J, et al. Quantitative multiplex methylation-specific PCR assay for the detection of promoter hypermethylation in multiple genes in breast cancer. Cancer Res 2004; 64:4442–52.
3. Fackler, M. J., Rivers, A., Wei, W. T., Mangat, A., Taylor, E., Zhang, Z., et al. (2009). Hypermethylated genes as biomarkers of cancer in women with pathologic nipple discharge. Clinical Cancer Research, 15(11), 3802-3811.
