Technical Details:
Nuclear lamina is a dense fibrillar network inside the nucleus of eukaryotic cells composed of intermediate filaments (lamins) and membrane associated proteins called lamin-associated membrane proteins (LAPs). The nuclear lamina provides mechanical support, regulates important cellular events such as DNA replication and cell division, participates in chromatin organization, and anchors the nuclear pore complexes embedded in the nuclear envelope. Lamin associated polypeptide 1 and 2 (LAP1, LAP2), emerin, lamin B-receptor (LBR), otefin and MAN1 are the most important LAPs mediating the attachment of the nuclear lamina to the nuclear envelope. LAP2 is a family of nucleoplasmic proteins that are expressed in various splicing isoforms that form oligomers and associates with chromosomes. LAP2 isoforms have been found to share a homologous D40 amino acid long region, the LEM domain, with nuclear membrane proteins MAN1 and emerin, which has been implicated in Emery- Dreifuss muscular dystrophy. A JHU scientist has generated Serum 2805, a polyclonal rabbit antibody against a recombinant polypeptide comprising amino acid residues 1-408 of human LAP2.
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A serum producing polyclonal rabbit antibodies against residues 1–408 of human LAP2 which can be used for immunoprecipitation.
