JHU scientists have generated a cell line incorporating a single base pair mutation “knocked in” within the AKT1 oncogene, single allele, into the MCF-10A human breast epithelial cell line resulting in a heterozygous E17K amino acid change. Additionally, a control “knock in” wild type cell line was also created, whereby gene targeting did not incorporate the mutation at the expected site. This serves as a further control in conjunction with the parental cell line.
Technical Details:
Akt1, is part of a protein family of serine/threonine-specific protein kinases known as Protein kinase B (PKB). These proteins regulate essential cellular functions such as differentiation, proliferation, apoptosis, migration and metabolism. Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes and inducing protein synthesis pathways. It is considered a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Because Akt1 can inhibit apoptosis and in so doing promote cell survival, it has been implicated as a major factor in many types of cancer.
Looking for Partners:
• This AKT1 cell line can be used as an oncogenic and biological research tool.
