C10643: Expression plasmids for HA-tagged FOXFl and HA-tagged FOXF2
Value Proposition:
Technical Details:
JHU scientists have developed HA-tagged FOXFl and HA-tagged FOXF2 expression plasmids. Full-length cDNAs for FOXF genes were isolated by using RT-PCR of RNA from normal mammary organoids and cloned them into pGEM-T-Easy vector (Promega). After sequencing confirmation of cloned FOXF cDNAs, the FOXF cDNA fragments were cut out from pGEM-T-Easy vector with EcoRI and ligated them with EcoRI-digested pcDNA3.1(–) (Invitrogen) to make pcDNA3.1-FOXF expression plasmids. For constructing haemagglutinin (HA)-tagged FOXF expression plasmids, the forward primer and its reverse complementary primer containing HA-tag coding sequence were synthesized, annealed, and then ligated with pcDNA3.1-FOXF expression plasmids pre-digested with XbaI and EcoRV. Primer sequences and conditions for cloning and construction are available on request.
Looking for Partners:
The constructed HA-tagged FOXF expression plasmid DNA can be used as research reagents.
Publications/Associated Cases:
Lo, P. K., & Sukumar, S. (2008). Epigenomics and breast cancer. Pharmacogenomics, 9(12), 1879-1902.
