Forkhead box-F (FOXF) Genes are Novel Negative Regulators of DNA Replication and are Epigenetically Silenced in Breast Cancer

Case ID:
C10643

C10643: Expression plasmids for HA-tagged FOXFl and HA-tagged FOXF2

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Technical Details:

JHU scientists have developed HA-tagged FOXFl and HA-tagged FOXF2 expression plasmids. Full-length cDNAs for FOXF genes were isolated by using RT-PCR of RNA from normal mammary organoids and cloned them into pGEM-T-Easy vector (Promega). After sequencing confirmation of cloned FOXF cDNAs, the FOXF cDNA fragments were cut out from pGEM-T-Easy vector with EcoRI and ligated them with EcoRI-digested pcDNA3.1(–) (Invitrogen) to make pcDNA3.1-FOXF expression plasmids. For constructing haemagglutinin (HA)-tagged FOXF expression plasmids, the forward primer and its reverse complementary primer containing HA-tag coding sequence were synthesized, annealed, and then ligated with pcDNA3.1-FOXF expression plasmids pre-digested with XbaI and EcoRV. Primer sequences and conditions for cloning and construction are available on request.

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The constructed HA-tagged FOXF expression plasmid DNA can be used as research reagents.


Publications/Associated Cases:

Lo, P. K., & Sukumar, S. (2008). Epigenomics and breast cancer. Pharmacogenomics, 9(12), 1879-1902.

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For Information, Contact:
Nakisha Holder
nickki@jhu.edu
410-614-0300
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