C10725: PC12 Stable Cell lines of Inducible Expression of Disrupted in Schizophrenia (DISC1)
Value Proposition: JHU inventors have produced neuronal (PC12) cell lines with inducible expression of DISC1. PC12 is a neuronal cell line derived from a pheochromocytoma of the rat brain. This technology includes an inducible Tet-off PC12 cell model with stable expression of full-length and truncated hDISC1, allowing for a regulated expression of hDISC1 in homogenous populations of cells.
Technical Details:
A balanced chromosomal translocation, segregating with mental illnesses in a large Scottish family, interrupts the disrupted-in-schizophrenia 1 (DISC1) gene, which would result in loss of DISC1 function via haploinsufficiency or dominant-negative effects (or possibly could cause gain-offunction effects) if a truncated protein is present. To evaluate the effects of a predicted protein, mutant DISC1, we generated stable PC12 cell clones with inducible expression of mutant or full-length human DISC1 (hDISC1). Inducible expression of DISC1 in PC12 cell clones is a valuable in vitro model for further studying the molecular mechanisms likely due to loss of function of DISC1 relevant to the pathogenesis of major mental illnesses.
Looking for Partners:
These stable cell lines can be commercialized as a research tool for study of schizophrenia disorder mechanisms and other neurological mechanisms.
