C11212: pET28a c/u Plasmid
Value Proposition:
• Bacterial vector with (URA3,Cen selection)
Technical Details:
BACKGROUND There is a wide range of vectors that are available to meet the various requirements for insertion, deletion alteration and expression of genes in yeast. Most of the plasmids used for yeast studies are shuttle vectors. Shuttle vectors contain sequences that permit selection and propagation in E. coli, thus allowing for convenient amplification and further alterations in vitro. In addition, all yeast vectors contain markers that allow selection of transformants containing the desired plasmid.
TECHNOLOGY JHU researchers have developed a plasmid that is a bacterial expression vector with 6 His-tag, derived from pET-28a. The vector has been modified to include a yeast URA3 and CEN selection to allow for homologous recombination cloning in yeast.
Looking for Partners:
Plasmid used for experiments for the insertion, deletion, alteration, and expression of genes in yeast.
Stage of Development:
Tangible Material
Publications/Associated Cases:
Reider, A., Barker, S. L., Mishra, S. K., Im, Y. J., Maldonado-Báez, L., Hurley, J. H., et al. (2009). Syp1 is a conserved endocytic adaptor that contains domains involved in cargo selection and membrane tubulation. EMBO Journal, 28(20), 3103-3116.
