Co-translational activation of a transcription factor by proteolytic cleavage

C11384: Novel Method for Real Time Monitoring of Gene Expression

Novelty:

The invention is a novel method which allows the targeted activation and characterization of a protein molecule of interest in a living cell without disrupting its natural state or functionality.

Value Proposition:

Currently, protein expression can be followed using translation fusion, however this risks disrupting the function of the protein. Another approach is using a reporter molecule on the same transcript or same promoter; however inaccuracy in counting undermines this approach. This technology addresses both of these shortcomings and allows for monitoring of gene regulation in real-time. Other advantages include:

Technical Details:

Johns Hopkins University researchers have developed compositions and methods to monitor gene regulation in real time. This method allows real time monitoring of the production of transcription factors as they are expressed in live E. coli cells. Moreover, the method allows the targeted activation and characterization of a protein molecule of interest in a living cell. The method is a translation fusion technology which ensures a one to one ratio of activated protein molecule and reporter molecule, thus providing readout of protein expression, activity, and quantity in real time without affecting the proteins native sequence and function.

Looking for Partners:

To commercialize the technology as a molecular research tool which includes reporter constructs and methods for the study of real-time quantitative protein expression and gene regulation in a living cell

Stage of Development:

Discovery

Data Availability:

Mebendazole significantly increases the average survival rate by 63% in the syngeneic and xenograft orthotopic mouse glioma models. Effective in animal models for a dosage level 75% under that considered safe in humans.

Publications/Associated Cases:

Nat Struct Mol Biol. 2012 Aug;19(8):797-802