Luminescent kinase activity biosensors

C11388: Novel Luminescent Biosensors to Visualize In Vivo Kinase Activity

Novelty:

Novel reporters designed to enable sensitive and robust real time visualization of protein kinase activity in vivo in either a population of cells or in a single cell.

Value Proposition:

Currently available, fluorescence resonance energy transfer (FRET)-based kinase activity reporters (KARs) are insensitive to important baseline variations in kinase activity. This technology consists of novel reporters that enable more sensitive and more robust detection of real time kinase activity in vivo. Other advantages include:

Technical Details:

Johns Hopkins University researchers have developed a new kinase inducible biomolecular switch (KIBS) that can be coupled to various reporting units to produce sensitive kinase activity reporters. The reporters are based on a platform for the design of kinase activity biosensors which are generalizable both in terms of kinase specificity and readout. This enables the reporters to easily adapt to detecting the activity of a specific kinase with various readouts. These newly developed reporters have been designed to reversibly determine real time dynamic activity of protein kinases in vivo in either a population of cells or in a single cell. These novel kinase activity biosensors will serve as powerful tools to facilitate sensitive and versatile detection of kinase activity in their native environments.

Looking for Partners:

To develop & commercialize the technology as an effective research tool to visualize kinase activity for adoption in the applications, including drug screening and research

Stage of Development:

Pre-Clinical

Data Availability:

Under CDA / NDA

Publications/Associated Cases:

J. Am. Chem. Soc., 2011, 133 (15), pp 5676–5679