C11456: E.coli Proteome Microarray ChipNovelty:
A protein microarray chip containing 4,176 proteins encoded by the E.coli K12 strain for proteomics studies in prokaryotes.
Value Proposition:
Proteome chips comprising of individually purified proteins are not common due to the complexity and difficulty of high-throughput protein purification. However for this invention, researchers have developed a high-throughput protein purification protocol that allowed them to purify 4,176 proteins encoded by the E.coli K12 strain. These proteins were then used to create E.coli proteome chips for analyzing biochemical activities of proteins at the proteomic level. Additional advantages include:
• Time-efficient protein analysis
• Easy protein interaction detection
• Minimum background
• Identification of novel enzymes or biomarkers from biological samples
Technical Details:
Johns Hopkins researchers have developed a miniaturized parallel assay system in the form of a proteome chip with individually purified E.coli proteins. 4,176 full-length proteins encoded by the E.coli K12 genome were purified via a novel high-throughput purification procedure and spotted onto glass slides as polyhistidine (His6) fusion proteins. Different surface chemistries were tested to confirm immobilization of the proteins onto the glass. Proteins on the chip are visualized useing anti-His6 antibodies or DyLight esters.
Looking for Partners:
To develop & commercialize the technology as a prokaryotic proteome chip for research use, or to utilize the chip for internal research use.
Stage of Development:
Data Validation
Data Availability:
Prototype
Publications/Associated Cases:
Nat Methods. 2008 Jan;5(1):69-74. Epub 2007 Dec 16
