C11676: PDGF Receptor Transgenic Mouse Line with Inducible Cre RecombinaseNovelty:
Transgenic mouse line containing inducible Cre recombinase expressed under the control of platelet derived growth factor alpha receptor (PDGF\R) promoter. This PDGFR-CreER mouse model is a useful resource for studying cell specific genetic regulation of oligodendrocytes in vivo.
Value Proposition:
Oligodendrocytes and pericytes in the central nervous system (CNS) express NG-2 and PDGFR. NG-2 promoters are commonly used to label NG2+ cells in transgenic models, but using a PDGFR promoter better restricts the transgene expression specifically to NG2+ cells. Therefore, this invention uses the PDGFR promoter to generate a transgenic mouse model with inducible Cre recombinase to study the fate of NG2+ cells in vivo. Other advantages include:
• In combination with other transgenic lines with floxed genes, particular gene expression can be regulated within specific cells
• Allows study of oligodendrocytes and their progeny in vivo
Technical Details:
Johns Hopkins researchers have generated a line of transgenic mice in which an inducible form of Cre recombinase is expressed under the control of the promoter PDGFR using the bacterial artificial chromosome (BAC) modification method. It is possible to induce or prevent expression of particular genes in these mice when used in combination with other transgenic models.
Looking for Partners:
To license the technology as a research tool for genetic studies of neuronal cells.
Stage of Development:
Ready for commercial use
Data Availability:
Under CDA / NDA
Publications/Associated Cases:
Neuron. 2010 Nov 18;68(4):668-81
C11675
C11677
C11678
