Novelty:
Unique transgenic mouse model comprising genetically encoded calcium indicator, GCaMP3, to monitor calcium signaling in vivo.
Value Proposition:
Commonly used calcium indicator dyes lack specificity and may produce error in measuring intracellular calcium. This invention is a transgenic mouse line with the genetically encoded calcium indicator, GCaMP3,that can be expressed in any cell that also expresses Cre for specific in vivo calcium studies. Other advantages include:
• GCaMP3 is sensitive to intracellular calcium and is ideal for studying in vivo cell activity
• Can be used to image cellular processes, such as action potentials
• High signal-to-noise ratio
• Stably expressed for months facilitating studies for longer periods
• Improved specificity over calcium indicator dyes
• Studies can be done for longer time periods in the same cells
Technical Details:
Johns Hopkins researchers have developed a line of transgenic mice (R26-lsl-GCaMP3) that allow selective expression of the genetically encoded calcium indicator GCaMP3 under the control of R26 promoter within different classes of cells.GCaMP3 is a fluorescent indicator created from the fusion of green fluorescent protein, calmodulin and M13. They are designed to show calcium ion status of a tissue or medium and are introduced into cell lines via transgenic crosses.
Looking for Partners:
To license the technology as a research tool for in vivo neuroscience or electro-physiological studies.
Stage of Development:
Ready for commercial use
Data Availability:
Under CDA / NDA
Publications/Associated Cases:
Corresponds to JAX stock #028764
Related to cases C11675, C11676, and C11678
