C11777: Chemically Inducible Dimerization System for Studying Signal TransductionNovelty:
The technology presents a novel research tool for manipulation of signal transduction using dimerization through modified gibberellins.
Value Proposition:
The technology is a novel system involving use of gibberellins analogs to manipulate and visualize protein signaling and perturbations through dimerization.
• Speed: The timescale is that of seconds which is hundreds of times faster than existing genetic circuits.
• Versatility: The system can provide several output signals including fluorescence, membrane ruffling, etc.
• The gibberellin system is completely orthogonal to the existing rapamycin-mediated dimerization system and thus suited for multivalent manipulation of different molecules at different locations.
• Logic Gates: AND/OR logic gates have been designed using both the rapamycin and gibberellin analog systems. This networked process may be applied in the future to higher-order computation.
Technical Details:
Johns Hopkins researchers developed a novel, efficient protein dimerization system using a chemically modified gibberellin analog and its binding proteins. The researchers developed and optimized a series of fusion proteins that form a dimerization system activated by a chemical, which readily enters mammalian cells and is cleaved to release the active analog. This gibberellin-mediated system is fully orthogonal to the rapamycin chemically induced dimerization system and can be used to induce protein translocation and to move active protein to specific subcellular locations on a timescale of seconds to minutes. Intracellular logic gates using two distinct inputs were generated by combining gibberellin- and rapamycin-based chemically induced dimerization systems.
Looking for Partners:
To develop and commercialize the technology as a research tool for studies on signal transduction.
Stage of Development:
Discovery
Data Availability:
Prototype
Publications/Associated Cases:
C11682
C11757
