Value Proposition
· Precisely Positioned: Vector contains tandem 601 nucleosome‑positioning for precise and predictable nucleosome positioning.
· Highly Stable: Produces nucleosome arrays with uniform spacing, delivering homogeneous samples ideal for biochemical, structural, and biophysical studies.
· Reproducible: by providing reproducible, standardized chromatin substrates, it accelerates assay development, improves data comparability across labs, and enables robust mechanistic and drug‑discovery research.
Unmet Need
· Researchers require DNA templates that yield consistent and predictable results to ensure experimental reproducibility across laboratories.
· A 601 array consists of tandem repeats of the 601 nucleosome positioning sequence, which strongly binds histones, is widely used to assemble uniformly spaced nucleosomes for structural, biochemical, and single-molecule studies.
· However, constructing long, repetitive 601 arrays is technically challenging. Repetitive DNA sequences are prone to spontaneous rearrangement or recombination in bacterial cloning systems, and achieving precise nucleosome spacing and defined copy number requires substantial time and technical expertise.
· The availability of a ready-made, sequence-verified, and stability-optimized vector would streamline this process, reduce experimental failure rates, and improve reproducibility, allowing researchers to focus on downstream analyses rather than complex cloning procedures.
Technology Description
· This bacterial DNA plasmid contains 34 repeats of the engineered “601” DNA sequence, repeated every 208 base pairs, and can be used in two ways:
1. Cut with EcoRI or EcoRV to release 208 bp fragments of DNA, with the 601 site either at the end (EcoRV) or in the center (EcoRI) of the fragment. This allows for the development of large quantities of DNA (milligrams), otherwise practically unattainable, that can be used for producing large quantities of nucleosomes for biochemical or biophysical experiments.
2. The entire 34-repeat sequence can be used as a single fragment, which when reconstituted with histones can make a long, evenly spaced string of nucleosomes. This model chromatin fiber can allow study of different factors, posttranslational modifications, and buffer conditions that may influence condensation/collapse of the chromatin fiber.
· The use of tandem 601 repeats enables predictable and reproducible nucleosome positioning, thereby improving experimental consistency and reducing the need to generate individual templates through PCR.
Stage of Development
· Fully developed
