Plasmid expression construct for the inorganic pyrophosphatase (PPase) gene of E. coli with its native promoter, Shine-Dalgarno sequence, and terminator.
The gene was PCR-amplified from E. coli K12 genomic DNA and cloned into pUC19, and the sequence of the insert was verified by
sequencing. The purified PPase is used as a coupling enzyme in a novel high throughout colorimetric assay to detect the tripolyphosphate
product (PPPi) of the enzyme human SAMHD1 when it converts dNTPs to dN and PPPi. Pyrophosphatase cleaves PPPi to 3Pi,
providing the required phosphate form for colorimetric detection.
Publications: J Biomol Screen. 2015 Jul;20(6):801-9.
