Polyclonal rabbit antibody against human recombinant Atox1

Case ID:

C14055

ABSTRACT
Atox1 is a 7.6 kDa cytosolic protein that plays an important role in human copper metabolism. The recombinant intein-fusion of Atox1 was expressed in E.coli, intein tag was cleaved during purification and purified Atox1 was used for immunization of rabbits. Serum containing anti-Atox1 antibodies was tested using mouse liver homogenates and purified Atox1. The anti-Atox1 antibody detects endogenous Atox1 and recombinant Atox1; it shows some cross reactivity in cell lysates with a non-specific 14 kDa band. Dilution 1:1000-1:2000.

FEATURES
Compared to the mouse monoclonal anti-Atox1 antibody currently available, this polyclonal anti-Atox1 antibody has higher sensitivity to endogenous Atox1 and can detect Atox1 in tissue lysates. It also detects Atox1 with chemically derivatized cysteines, whereas current antibody does not.

MAJOR RESULTS

•The antibody can be used for detection of endogenous and recombinant Atox1 by Western blotting.

ASSOCIATED INVENTORS
Physiology, School of Medicine
PI: Svetlana Lutsenko, Ph.D.
Yuta Hatori, Ph.D

ASSOCIATED PUBLICATIONS
■ Nat Commun. 2016 Feb 16;7:10640. ■
■ J Biol Chem. 2012 Aug 3;287(32):26678-87 ■

Direct Link:

https://jhu.technologypublisher.com/technology/22748

Inventors:

Category(s):

Technology Classifications > Research Tools > Animal Models, Technology Classifications > Research Tools, Technology Classifications > Research Tools > Antibodies,

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For Information, Contact:

Christine Joseph

cjoseph6@jhmi.edu

410-614-0300

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