TITLE
Polyclonal chicken and rabbit antibodies, termed anti-CC, of mouse polycystin-1 protein as immunogen
C13399
ABSTRACT
Polycystin-1 (PC1) has an essential function in renal tubular morphogenesis and disruption of its function causes cystogenesis in human autosomal dominant polycystic kidney disease. Endogenous polycystin-1 is expressed at very low level and is difficult to be immunoprecipated or detected by common antibodies. JHU researcher have generated a set of anti-CC antibodies that can be used to immunoprecipitate (IP) and detect endogenous PC1 proteins (chicken anti-CC for IP, and rabbit anti-CC for Western blot detection).
FEATURES
Johns Hopkins researcher generated the polyclonal CC antibody using a denatured C-terminal 199 amino acid fragment of mouse polycystin-1 protein as immunogen. After cloning and expression the protein was affinity-purified and then used to immunize rabbits and chickens. The purified chicken anti-CC was used for IP and rabbit anti-CC was used for western blot detection.
STAGE OF DEVELOPMENT
Tangible material,
1. Chicken anti-mouse Polycystin-1 (PC1), C-Terminal Polyclonal Antibody.
2. Rabbit anti-mouse Polycystin-1 (PC1), C-Terminal Polyclonal Antibody
DISEASE INDICATION
Polycystic Kidney Disease
ASSOCIATED PUBLICATIONS
- Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18688-93 -
TECHNOLOGY CLASSIFICATION
Primary Category: Discovery/Research Tools
Primary Subcategory: Research Reagent
