TITLE
CCL20 Reporter and Conditional Knockout Mice
CASE NUMBER
C13357
ABSTRACT
CCL20 a member of the macrophage inflammatory protein family and signal through the receptor CCR6, genomic location have been identified as a region important for bone quality. However, the role of CCL20 signaling in regulating bone mass is unknown. Johns Hopkins researcher have created mouse models to aid in determining the role of the chemokine CCL20 in bone formation and remodeling.
FEATURES
Johns Hopkins researchers have developed the first mice that allows visualization of cells expressing CCL20 and mic allowing or harboring a condition deletion of CCL20. To allow visualization of cells expressing CCL20, CCL20tm1a mice were crossed with Cre-deleter mice, resulting in mice that express the reporter gene lacZ in cells where the CCL20 promoter is active. Staining tissues from these mice with beta-galactosidase permitted the timing and location of CCL20 expression to be determined. To achieve conditional deletion of CCL20, CCL20tma1 mice were crossed with Flp-deleter mice, resulting in conditional-ready CCL20flox/flox mice. CCL20flox/flox mice can subsequently be crossed with mice expressing Cre under the control of a specific gene promoter (eg. osteocalcin), thus permitting cell-restricted deletion of CCL20 per Cre-lox technology.
Individual models created will allow:
1. identification of cells in which CCL20 is expressed and
2. deletion of CCL20 expression in specific cell types.
3. Examination of these mice will allow the biological impact of CCL20 to be determined as well as its mechanism of action.
4. These will be of interest to the field of Immunology or inflammatory diseases (eg. rheumatoid arthritis, asthma, psoriasis, etc.) where CCL20 is believed to play a role.
STAGE OF DEVELOPMENT
Tangible material,
CCL20lacZ mice
CCL20flox/flox mice
CCL20gene X-Cre mice
DISEASE INDICATION
Bone Disorders, Inflammatory Disorders
ASSOCIATED PUBLICATIONS
- J Bone Miner Res. 2016 Jul;31(7):1381-90 -
