Human Mesothelin Expressing ID8-Defb29 Vegf/Luc (ID8-Defb29 Vegf-Luc/Hmeso)

ABSTRACT
Cancer immunotherapy targeting mesothelin represents a potentially plausible approach for the control of ovarian cancer as most ovarian cancers express high levels of mesothelin. In the current study, JHU researchers created a mesothelin-positive luciferase-expressing ovarian cancer model, ID8-Defb29 Vegf /luc. ID8-Defb29 Vegf –luc was infected by retrovirus encoding human mesothelin. ID8-Defb29 Vegf-luc/Hmeso was isolated by preparative flow cytometry of stained cells with human mesothelin antibody.
 
FEATURES
The ID8-Defb29 Vegf -luc cell line is generated by infecting the ID8-Defb29 Vegf cell line, a preclinical ovarian cancer cell line with retrovirus that contains a plasmid encoding firefly luciferase and thy1.1. The resulted ID8-Defb29 Vegf -Luc tumor cell line constantly express firefly luciferase, which allows for the monitoring of tumor growth as well as the assessment of the effects of tested therapeutic strategies in controlling tumor in tumor bearing mice through luminescence imaging. The ID8-Defb29 Vegf-luc/Hmeso cell line is generated by infecting the ID8-Defb29 Vegf-luc cell line with retrovirus that contains a plasmid encoding human mesothelin. The resulted ID8-Defb29 Vegf-luc/Hmeso tumor cell line constantly express firefly luciferase and human mesothelin, which allows for the monitoring of tumor growth as well as the assessment of the effects of tested mesothelin specific therapeutic therapies in controlling tumor in tumor bearing mice through luminescence imaging.
 
STAGE OF DEVELOPMENT
Tangible material:  ID8-Defb29 Vegf –Luc/Hmeso tumor cell line
 
ASSOCIATED INVENTORS
Pathology, School of Medicine
PI: T.C. Wu, M.D., Ph.D.
Chien-Fu Hung, Ph.D.

ASSOCIATED PUBLICATIONS
■  PMC3183576 ■

 

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