Development of a novel antibody to the Estrogen Receptor beta (ER-beta)
JHU REF: C13125
Invention novelty: This tangible material is a genetically modified GnRH neuron-specific ER-beta knockout mouse model (GER-beta-KO) and a specific antisera for mouse ER-beta (mER-beta) that can be used to explore the cellular and physiological role of ER-beta.
Value Proposition
Two estrogen receptors (ERs) have been identified to date ER-alpha and ER-beta. Although much is known about ER-alpha at the mRNA and protein levels, our knowledge of the expression and distribution of ER-beta protein is much more limited. Current available antibodies for ER-beta are not very reliable due to the divergent immunohistochemical analysis results. This developed novel mER-beta antisera addresses this lack of specific antisera against mER-beta protein. This polyclonal antibody will allow investigators to explore the cellular and physiological role of ER-beta.
Technical Details
Johns Hopkins researchers have developed a genetically modified mouse model GER-beta-KO and antisera for mER-beta. The antisera was developed using 18 specific amino acids (CSTEDSKSKEGSQNLQSQ) in the C-terminus of the mER-beta conjugated to keyhole limpet hemocyanin protein. The antisera was then used to confirm to the ER-beta deletion in the GnRH neurons of GER-beta-KO mice.
Stage of Development: Tangible Material
Data Availability: Animal data
Patent Status: None – Tangible Material
Publication(s)/Associated Cases: OR30-2: Reproductive Axis Disruption of Mice Lacking Estrogen Receptor β in GnRH Neurons
Categories: Research Tools, Tangible Property, Mouse strain
Keywords: Estrogen receptor beta; ER-beta antisera; knockout mice
