Development of a Novel Antibody to the Estrogen Receptor Beta (ERbeta)

Case ID:
C13125

Development of a novel antibody to the Estrogen Receptor beta (ER-beta)

JHU REF: C13125

 

Invention novelty: This tangible material is a genetically modified GnRH neuron-specific ER-beta knockout mouse model (GER-beta-KO) and a specific antisera for mouse ER-beta (mER-beta) that can be used to explore the cellular and physiological role of ER-beta.

 

Value Proposition

Two estrogen receptors (ERs) have been identified to date ER-alpha and ER-beta. Although much is known about ER-alpha at the mRNA and protein levels, our knowledge of the expression and distribution of ER-beta protein is much more limited. Current available antibodies for ER-beta are not very reliable due to the divergent immunohistochemical analysis results.  This developed novel mER-beta antisera addresses this lack of specific antisera against mER-beta protein. This polyclonal antibody will allow investigators to explore the cellular and physiological role of ER-beta.

 

Technical Details

Johns Hopkins researchers have developed a genetically modified mouse model GER-beta-KO and antisera for mER-beta. The antisera was developed using 18 specific amino acids (CSTEDSKSKEGSQNLQSQ) in the C-terminus of the mER-beta conjugated to keyhole limpet hemocyanin protein. The antisera was then used to confirm to the ER-beta deletion in the GnRH neurons of GER-beta-KO mice.

 

Stage of Development: Tangible Material

 

Data Availability: Animal data

  

Patent Status: None – Tangible Material

 

Publication(s)/Associated Cases: OR30-2: Reproductive Axis Disruption of Mice Lacking Estrogen Receptor β in GnRH Neurons

 

Categories: Research Tools, Tangible Property, Mouse strain

Keywords: Estrogen receptor beta; ER-beta antisera; knockout mice

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For Information, Contact:
Christine Joseph
cjoseph6@jhmi.edu
410-614-0300
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