A Surface Plasmon Resonance Approach to Monitor Animal Toxin Interactions with Isolated Voltage-gated Sodium Channel Paddle Motifs and the Use Thereof

Case ID:

C13230

Animal toxins that inhibit voltage-gated sodium (Nav) channel fast inactivation can do so through an interaction with the S3b-S4 helix-turn-helix motif, or paddle motif, located in the domain IV voltage sensor. Here, we show that the domain IV paddle can be lifted out of the Nav channel and immobilized on sensor chips. Subsequent Surface Plasmon Resonance (SPR) experiments suggest that this motif retains its susceptibility to animal toxins that target the domain IV voltage sensor. As such, our results reveal the inherent pharmacological sensitivities of the isolated paddle motif which may be exploited to develop label-free SPR screens for novel ligands that target this region.

Patent Information:

Title	App Type	Country	Serial No.	Patent No.	File Date	Issued Date	Expire Date	Patent Status
Surface Plasmon Resonance Approach to Monitor Protein-Ligand Interactions	PCT: Patent Cooperation Treaty	United States	15/545,980	10,429,300	7/24/2017	10/1/2019	1/26/2036	Granted

Direct Link:

https://jhu.technologypublisher.com/technology/24073

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Category(s):

Technology Classifications > Diagnostics > In Vitro Diagnostics, Technology Classifications > Research Tools, Technology Classifications > Diagnostics, Technology Classifications > Research Tools > Assays,

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For Information, Contact:

Vera Sampels

vsampel2@jhu.edu

410-614-0300

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