Single Molecule Free Solution Hydrodynamic Separation Platform

A method of separating, detecting and determining a size of each of a plurality of particles in a fluid includes compelling the fluid to flow through a fluid channel such that larger particles of the plurality of particles travel through the fluid channel faster than smaller particles of the plurality of particles; illuminating a detection zone of the fluid channel substantially uniformly across an entire cross section of the fluid channel such that each of the plurality of particles passes through illumination light upon passing through the detection zone; detecting each of the plurality of particles based on corresponding responses to the illuminating to determine a time that each of the plurality of particles passes through the detection zone; and determining a size of each of the plurality of particles based on the time that each of the plurality of particles passes through the detection zone.

 

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