Guiding the Derivation of Endothelial Cells from Human Pluripotent Stem Cells in a Two-dimensional, Feeder Free Differentiation Scheme

New defined chemical method for growth of endothelial cells from human stem cells

JHU REF: [C11924]

Invention novelty: Development of defined chemical conditions and method for culturing of endothelial cells (ECs) from human embryonic and induced pluripotent stem cells using sequential adherent cell culture method.
 
Value Proposition
The development of a defined chemical method for the culturing of homogeneous populations of endothelial cells from human stem cells overcomes the following problems faced in the current structural tissue culturing practices:

1. Thrombotic blockage of synthetic polymer grafts of diameters less than 4 mm
2. Difficulty in functionalizing and maintaining the mechanical properties of endothelial cells in in vivo systems.
3. Lack of defined technique for obtaining homogeneous endothelial cell cultures from human embryonic and induced pluripotent stem cells.

Technical Details:
Johns Hopkins University researchers have  defined the chemical conditions for the controlled differentiation and derivation of functional Endothelial Cells (ECs) from human embryonic stem cell line (hESCs) H9 and the induced pluripotent stem cell line (hESCs) MR31. This is done using adherent cell culture method and the homogeneity of Endothelial cells obtained from hESCs and hPSCs  was observed by comparing its growth in 2 dimensional sequential scheme and directed scheme. Using this chemical composition, it was observed that sequential method of differentiation was conducive in comparison to direct differentiation method for the culturing of endothelial cells. The cells derived using the defined chemical medium and technique exhibited the appropriate marker profiles and optimum functionality.This invention thereby establishes an efficient and clinically relevant methodology for deriving functional endothelial cells from stem cells.
 
Looking for Partners: To develop & commercialize the technology as a method for the culturing of endothelial cells from human stem cells for tissue engineering applications.
 
Stage of Development: Prototype
 
Data Availability: Animal data 
 
Patent Status: Provisional  
 
Publication(s): Not at this time
 

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