SUB280 Yeast with Hygromycin Marker at LEU2

Case ID:

C12803

An Ubiquitin Expressed Yeast Strain at LEU2

JHU REF: [C12803]

Invention Novelty: A genetically engineered single ubiquitin expressed Yeast strain at (leucine) LEU2 to serve as an in vivo model for the discovery of ubiquitin linkage roles in subcellular signal pathways

Value Proposition: Unique Polyubiquitination (polyUb) linkage combinations serve essential roles in several nondegradation pathways that dictate substrate fate within the cell, but due to insufficient in vivo models and ubiquitin’s dynamic nature, little is known about ubiquitin linkage dependent pathways. This technology is a single ubiquitin expressed yeast model at LEU2 which can serve as an in vivo research model to further examine Lys63-polyUb specific relationships and analysis of its conjugates. Other advantages include:

to assist in yeast strain identification within culture

Models have similar signal patterns to human cells

Specific analysis of individual Ub transgene relationships

Discovery of novel Poly-Ub chain subcellular dynamics

Technical Details: Johns Hopkins researchers have developed a single ubiquitin expressed mutant yeast model at LEU2 in order to provide an in vivo research tool to identify the role of Poly-Ub linkage combinations in nonproteasomal degradation pathways. Inventors used molecular genetic engineering techniques to replace UB14 gene with LEU2 inducing a hygromycin resistance which can serve as a marker or assist in strain identification. This yeast model overall will facilitate study of Lys63-polyUb specific linkages and its protein conjugates.

Looking for Partners: To develop and commercialize the technology as a tool to model ubiquitin- dependent regulated subcellular pathways and study its linkage- specific relationships

Stage of Development: Ready to Use

Data Availability: Proof of Principal

Patent Status: Tangible Material

Publication(s)/Associated Cases: Nature Methods 9,303–309 (2012)

Direct Link:

https://jhu.technologypublisher.com/technology/24317

Inventors:

Category(s):

Technology Classifications > Research Tools > Cell Lines, Technology Classifications > Research Tools, Technology Classifications > Research Tools > Yeast Model,

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For Information, Contact:

Christine Joseph

cjoseph6@jhmi.edu

410-614-0300

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