Phosphatidylinositol 3-kinase (alpha) Expression Vectors

ABSTRACT
Researchers at Johns Hopkins University have developed a set of plasmids that express recombinant P110a/niP85a heterodimer providing the means to purify large quantities of a fusion protein containing the full length phosphatidylinositol 3 kinase alpha (P110α) in complex with residues 322-600 of the regulatory subunit p85.  The fusion protein has an amino-terminal tag for purification.

FEATURES
The recombinant heterodimers expressed using these plasmids can be used in in vitro assays and structural biology experiments. These plasmids provide an active and stable PI3Ka enzyme wild type or PI3Ka H1047R mutant.

STAGE OF DEVELOPMENT
1. Plasmid 16641: PIK3CA - Exon9 H1047R (pFastBac)  - (http://www.addgene.org/16641/)

2. Plasmid 16640: PIK3CA - Exon20 E545K (pFastBac) - (http://www.addgene.org/16640/)

3. Plasmid 17744: pFastBac1 PIK3R1-Ni (http://www.addgene.org/17744/)

ASSOCIATED INVENTORS
Biophysics, A & S; Oncology, School of Medicine
PI: Sandra B. Gabelli, M.D., Ph.D.
Bert Vogelstein, M.D.

ASSOCIATED PUBLICATIONS
■  Science. 2004 Apr 23. 304(5670):554. ■
■  Science. 2007 Dec 14;318(5857):1744-8 ■
■ Proc Natl Acad Sci U S A. 2009 Oct 6;106(40):16996-7001 ■




 

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