UNMET NEED
An emerging concern in the field of cancer pathology is the reliability and reproducibility of immunohistochemical (IHC), in situ hybridization (ISH), and fluorescence in situ hybridization (FISH) methods to evaluate gene expression and genome alterations in tissues specimens. A tool that could be used to standardize the parameters of IHC or ISH for specific molecular targets would thus be very valuable for biomarker discovery and validation.
PROBLEM SOLVED
Nanobodies are single chain antibodies that can be used for fundamental and clinical research. We have produced, purified, and characterized the panel of nanobodies against the metal-binding domain of a human copper transporter ATP7B (Wilson disease protein) and demonstrated that several of these nanobodies can be used for protein detection in immunochemical assays and in cells. We have also demonstrated that nanobodies can be derivatized without loss of their activity.
MAJOR RESULTS
1. ATP7B has been characterized using traditional polyclonal antibody;
2. nanobody is a novel reagent that substitutes anti-ATP7B antibodies at a fraction of a cost.
3. In addition, unlike traditional antibodies, the nanobody can be further derivatized which increases the range of the application.
DISEASE INDICATION
Wilson Disease
ASSOCIATED PUBLICATIONS■
J Biol Chem. 2014 Nov 21;289(47):32682-93 ■
TECHNOLOGY CLASSIFICATION
Primary Category: Research Reagent
Primary Subcategory:
