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Early Cancer Detection by Single-molecule Imaging of Nucleocytoplasmic Tumor-specific Proteins in the Peripheral Circulation
Case ID:
C13781
Report of Invention:
8/13/2015
Web Published:
3/17/2017
Unmet Need / Invention Novelty:
Due to lack of effective early detection modalities, ovarian and pancreatic cancers are rarely found until the disease has progressed and hence are highly lethal. To overcome these limitations we report an ultra-sensitive microfluidic chip designed to bind and digitally quantify tumor-derived cancer proteins for an early disease detection and improved patient outcome.
Technical Details:
Johns Hopkins researchers have developed SMAC (single-molecule analysis-and counting), a chip-based platform for the ultra-sensitive detection and quantification of nucleocytoplasmic proteins to diagnose early-stage cancer. SMAC integrates microfluidic herringbone structures coated with capture molecules that bind tumor-specific biomarkers found in patient serum. Target oncoproteins are concentrated using high velocity, closed-loop flow through the SMAC chip (actuated by a pump) that are then digitally imaged using fluorescence microscopy. These bound molecules are automatically quantified by a computer algorithm that discriminates between specific and non-specific binding events and calculates the number of fluorescence spots that score above a set threshold to provide ultra-sensitive early cancer detection. Preclinical trials using tumor-bearing and naïve control mice suggests that SMAC analysis of tumor-derived nucleoplasmic proteins can detect early stage cancer with 100% specificity and sensitivity.
Value Proposition:
No need for biochemical signal amplification due to ultra-high sensitivity capture of target proteins on chip
Entirely new way of bonding microfluidic devices suitable for SMD
Digital molecule counting algorithm discriminates between on-off target detection
SMAC analysis of tissue restricted biomarkers can indicate the anatomical location of the tumor
Looking for Partners to:
Develop & commercialize the technology as early-cancer detection modality.
Stage of Development
: Pre-clinical
Data Availability:
Validated in multiple preclinical mouse models
Inventors:
T.C. Wu, Chih-Ping Mao, Shih-Chin Wang, Jie Xiao, Chien-Fu Hung
Patent Status:
Patent Pending
US-2019-0308190
Publication(s):
S.C. Wang, C.P. Mao, Y.P. Su, T.C. Wu, C.F. Hung, J. Xiao. Protein Detection in Blood with Single-Molecule Imaging.
Biophys
116 (3), 465a
Patent Information:
Title
App Type
Country
Serial No.
Patent No.
File Date
Issued Date
Expire Date
Patent Status
NON-INVASIVE CANCER DETECTION AND ANALYSIS BY SINGLE-MOLECULE IMAGING
CON: Continuation
United States
18/437,439
2/9/2024
Pending
Direct Link:
https://jhu.technologypublisher.com/technology/24859
Inventors:
Category(s):
Clinical and Disease Specializations, Clinical and Disease Specializations > Oncology, Technology Classifications > Diagnostics > Biomarkers, Technology Classifications > Diagnostics > Diagnostic Biomarkers, Technology Classifications > Diagnostics > In Vitro Diagnostics, Technology Classifications > Research Tools, Technology Classifications > Diagnostics,
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For Information, Contact:
Vera Sampels
vsampel2@jhu.edu
410-614-0300
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