Hypoxia-inducible factor reporter cell lines

Unmet Need
Low oxygen levels are a characteristic of many types of solid tumors. High levels of HIF1α, the transcription factor responsible for energy utilization under hypoxic conditions, has been implicated in tumor growth and resistance, angiogenesis, and poor patient prognosis. For these reasons, HIF1α remains an attractive target for novel cancer therapeutics. The field currently lacks robust tools for high-throughput screening of candidate compounds to target this pathway.
 
Technology Overview
Researchers at Johns Hopkins have used a lentiviral construct to engineer multiple human HIF-1α reporter cell lines. These cell lines can be used to identify modulators of oxygen homeostasis, energy utilization, erythrogenesis, and cancer metabolism.
 
Stage of Development
The inventors have engineered human cell lines with a robust GFP reporter of HIF1α activity, and have validated their reporting capabilities using in vitro assays.
 
Publications
None at this time.
 

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