TITLE: TC-1/A2 cell line
CASE NUMBER: C04953
TECHNOLOGY
JHU Scientists characterized the ability of DNA-encoding calreticulin linked to DNA-encoding E7 antigen to generate HLA-A2-restricted E7-specific CD8+ T-cell responses in HLA-A2 (AAD) transgenic mice, as well as antitumor effects against an E7+ HLA-A2+ tumor cell line, TC-1/A2.
TECHNICAL DETAILS
Scientists at JHU transduced TC-1, an established E7-expressing tumor cell line, with a retrovirus encoding the HLA-A2 (AAD) molecule. The expression of HLA-A2 in the TC-1/A2 cells was confirmed by flow cytometry analysis using an antibody against HLA-A2. Characterization of HLA-A2 (AAD)-expressing TC-1 cells (TC-1/A2) was done by:
(a) Intracellular cytokine staining followed by flow cytometry analysis to determine activation of E7 (aa11–20)-specific CD8+ T cells by TC-1/A2 cells and,
(b) CTL assay to demonstrate specific killing of TC-1/A2 cells by E7 (aa11–20)-specific CD8+ T cells.
TC-1/A2, but not TC-1, was able to activate HLA-A2-restricted E7 (aa11–20)-specific CD8+ T cells in splenocytes from pcDNA3-CRT/mtE7 (del aa49–57)-vaccinated HLA-A2 (AAD) transgenic mice. CTL assays showed specific lysis occurred in TC-1/A2 cells but not in TC-1 cells. The data suggest that TC-1/A2 tumor cells are capable of processing and presenting the E7 (aa11–20) epitope through HLA-A2 molecules.
STAGE OF DEVELOPMENT
Tangible material TC-1/A2 cell line available
ASSOCIATED INVENTORS
Pathology, School of Medicine
PI: T.C. WU, M.D., Ph.D.
Chien-Fu Hung, Ph.D.
ASSOCIATED PUBLICATIONS
■ Peng et al. 2006 “Characterization of HLA-A2-restricted HPV-16 E7-specific CD8 (+) T-cell immune responses induced by DNA vaccines in HLA-A2 transgenic mice”. Gene Ther. 13(1):67-77.■
CONTACT INFORMATION
Christine Joseph
Technology Analyst, Life Sciences Licensing
Johns Hopkins Technology Ventures
410-502-8355
cjoseph6@jhmi.edu
