Unmet Need: Acylglycerol kinase, AGK, is a protein in the TIMM22 translocon and is postulated to be important in cardiolipin biogenesis. Mutations in the gene encoding for AGK also can result in human disease, making the study of the protein important in understanding disease pathogenesis. Currently there are no validated human AGK specific antibodies, a necessary research tool.
Technical Details: Johns Hopkins researchers developed and validated a monoclonal antibody that detects human AGK. Using a purified recombinant human AGK as an antigen, researchers created five hybridoma cell lines that secrete AGK antibodies in cellular extracts. The monoclonal antibodies were shown to specifically detect endogenous AGK as verified by immunoblot.
Value Proposition:
· Validated monoclonal antibody against human AGK
· Detects endogenously expressed protein with high specificity
· Five different AGK antibody isotypes generated by AGK-specific hybridomas
· Suitable for immunoblot, immunocytochemistry, immunoprecipitation and other research applications
Looking for Partners to: Develop and license the human AGK monoclonal antibody for research purposes
Stage of Development: Cell lines are available.
Data Availability: In Vitro
Publication(s): Acoba MG, Alpergin ESS, Renuse S, Fernández-Del-Río L, Lu YW, Khalimonchuk O, Clarke CF, Pandey A, Wolfgang MJ, Claypool SM. The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism. Cell Rep. 2021 Mar 16;34(11):108869. doi: 10.1016/j.celrep.2021.108869. PMID: 33730581; PMCID: PMC8048093.
