Marketing Summary Draft

Inducible OGT Knock-out Cell Line

JHU Ref #: C17974

Value Proposition

·        Mouse embryonic fibroblast cell line

·        Rapid and reproducible depletion of essential OGT gene

·        Enables O-GlcNAc modification studies

Unmet Need

·        O-GlcNAcylation (O-GlcNAc) is a nutrient- and stress-responsive post-translational modification involved in the regulation of various cellular processes such as DNA transcription, epigenetic modifications and cell signaling dynamics (Yang). Disruption to O-GlcNAc homeostasis is implicated in the pathogenesis of many human diseases, including cancer, diabetes, and neurodegeneration. Currently, the mechanisms underlying the spatiotemporal control of O-GlcNAc signaling are largely unknown. Thus, the development of research tools to investigate the dynamics of O-GlcNAc is necessary.

Technology Description

·        The O-GlcNAc transferase, OGT, is an essential gene responsible for the attaching the O-GlcNAc modification to proteins at specific serine or threonine residues. JHU researchers have developed a mouse embryonic fibroblast (MEF) cell line able to rapidly deplete OGT following treatment by 4-Hydroxutomoxifen (4HT). The researchers were able to use their knock-out cell line to study the O-GlcNAc-mediated regulation of heat shock protein expression.

Stage of Development

·        Studies have been published.

Data Availability

·        Data available at below publication.

Publication

https://doi.org/10.1074/jbc.M110.131102