Polymers for Binding-Triggered Antibody Precipitation and Purification

Case ID:
C16521

Value Proposition

  • Results in >97% precipitation yield, >88% elution yield of mAbs
  • Simplified molecular design compared to elastin-like polypeptide (ELP)-based materials
  • Does not need additional salt to achieve mAb precipitation and recovery

Technology Description

Researchers at Johns Hopkins have developed a peptide-based platform technology using supramolecular immunofibers (IFs) for non-chromatographic antibody purification. The platform employs co-assembly of filler and ligand molecules. In aqueous solutions, the filler and ligand molecules can spontaneously co-assemble into IFs under physiological conditions and then capture and precipitate monoclonal antibodies (mAbs).


Unmet Need

Antibody precipitation using protein A chromatography is one of the major downstream bottlenecks due to resin capacity limitation and high production cost. Affinity precipitation has been increasingly explored as a promising alternative to purify mAbs. However, high salt concentration is known to increase the risks of protein instability and non-specific precipitation of impurities present in the solution. Therefore, there is a need for an alternative mAb purification process of with high throughput, ease of handling, and reduced cost.


Stage of Development

  • In vitro studies have been completed and demonstrate high precipitation yields and elution yields of mAbs for high titers
  • Current work is evaluating process scalability, robustness, and process cost, time, and efficiency.

Data Availability

Data available upon request.

Patent Information:
Title App Type Country Serial No. Patent No. File Date Issued Date Expire Date Patent Status
HIGH-AFFINITY SUPRAMOLECULAR POLYMERS FOR BINDING-TRIGGERED ANTIBODY PRECIPITATION AND PURIFICATION PCT: Patent Cooperation Treaty European Patent Office 22846765.0   7/18/2022     Pending
HIGH-AFFINITY SUPRAMOLECULAR POLYMERS FOR BINDING-TRIGGERED ANTIBODY PRECIPITATION AND PURIFICATION PCT: Patent Cooperation Treaty Korea (South) 10-2024-7004948   7/18/2022     Pending
HIGH-AFFINITY SUPRAMOLECULAR POLYMERS FOR BINDING-TRIGGERED ANTIBODY PRECIPITATION AND PURIFICATION PCT: Patent Cooperation Treaty Japan 2024-501249   7/18/2022     Pending
HIGH-AFFINITY SUPRAMOLECULAR POLYMERS FOR BINDING-TRIGGERED ANTIBODY PRECIPITATION AND PURIFICATION PCT: Patent Cooperation Treaty China 202280051543.9   7/18/2022     Pending
HIGH-AFFINITY SUPRAMOLECULAR POLYMERS FOR BINDING-TRIGGERED ANTIBODY PRECIPITATION AND PURIFICATION PCT: Patent Cooperation Treaty United States 18/579,578   1/16/2024     Pending
Inventors:
Category(s):
Get custom alerts for techs in these categories/from these inventors:
For Information, Contact:
Vera Sampels
vsampel2@jhu.edu
410-614-0300
Save This Technology:
2017 - 2022 © Johns Hopkins Technology Ventures. All Rights Reserved. Powered by Inteum