Multi-Compartment Fallopian Tube Organoid Model

Unmet Need: Existing organoid models of the fallopian tube are architecturally simplified compared to the intricate epithelial-lumen interface in scale and structure, and there remains a need in the art for multi-compartment fallopian tube organoids that architecturally simulate the intricate architecture of the epithelial-lumen interface in structural complexity, scale, and composition, as well as the tissue’s molecular expression patterns and function. Disclosed here are methods and compositions to create multi-compartment organoids that are an in vitro 3-dimension (3D) physiologically accurate model of a tissue or organ.

• Multi-compartment physiologically-accurate organoid model
• Decreased volume of reagents, resulting in streamlined organoid creation
• Suitable for co-cultures to simulate tissue structure in physiologically relevant conditions
• Powerful 3D culture tool to facilitate drug discovery applications in women’s health and other disease indications

Technology Overview: Johns Hopkins researchers have developed a pipeline that uses oil-in-water droplet microtechnology to reliably and reproducibly generate small volume, physiologically-relevant organoids for drug discovery applications. The multi-compartment design increases physiological relevance by thorough recapitulation of tissue architecture, function, molecular expression and allows for relevant cell-extracellular matrix and cell-cell interactions. The pipeline also reduces total reagents required to establish 3D culture from primary or immortalized cell lines.

Stage of Development: Organoid platform established and validated