Multiplex digital PCR diagnostic system
JHU Ref #: C18050
Value Proposition:
· The use of color-specific and temperature-specific probes allows for unique molecular signals during digital PCR reaction
· Can detect low abundance disease biomarkers in a variety of disease settings
· Enhanced throughput and sensitivity compared to current methods
· Can simultaneously detect up to 66 targets
Technology Description
· Currently there are many multiplex detection methods that are available for use in biomedical research but relatively few in the diagnostic space and the available methods often rely on expensive, complicated protocols and equipment. Johns Hopkins researchers have developed a multiplex digital PCR method for the simultaneous detection of up to 66 nucleic acid or protein targets. The method combines a multi-color, multi-temperature approach to distinguish between target analytes and enables a fast, high throughput approach for disease diagnostics and research settings.
Unmet Need
· Detection of multiple target molecules in a biological sample provides researchers and clinicians with the ability to simultaneously screen for multiple pathogens, diagnose the presence of complex diseases with low abundance markers, and evaluate the breadth of immune responses.
· Currently there are many multiplex detection methods that are available for use in biomedical research but relatively few in the diagnostic space and these methods often rely on expensive, complicated protocols and equipment.
· Therefore, there is a strong need for new highly sensitive, low-cost multiplex methods to be developed for disease diagnosis which can be performed quickly and easily.
Stage of Development
· Preclinical assay development
Publication
· N/A
