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A CRISPR-based yeast two-hybrid system for investigating RNA-protein interaction

Unmet Need: DNA-sequencing technology has led to the development of many powerful techniques, such as RIP-seq and CLIP-seq, which can be used to identify many RNAs bound by a protein of interest, however, few protocols exist for identifying the proteins bound to a particular RNA. Most available techniques involve RNA pull-down followed by protein identification...

Published: 3/13/2025 | Inventor(s): David Zappulla, Evan Hass

Keywords(s): Analytical Method, Assay, Clinical Diagnostics, Discovery/Research Tools, In Vitro Diagnostics, In Vitro Research Tool, Target-based Screening Tool, Yeast Model

Category(s): Technology Classifications > Research Tools > Cell Lines, Technology Classifications > Research Tools, Technology Classifications > Diagnostics > In Vitro Diagnostics, Technology Classifications > Research Tools > Assays, Technology Classifications > Diagnostics