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Expression Plasmid pUC19-PPase for E. coli Pyrophosphatase

Plasmid expression construct for the inorganic pyrophosphatase (PPase) gene of E. coli with its native promoter, Shine-Dalgarno sequence, and terminator. The gene was PCR-amplified from E. coli K12 genomic DNA and cloned into pUC19, and the sequence of the insert was verified by sequencing. The purified PPase is used as a coupling enzyme in a novel...

Published: 3/13/2025 | Inventor(s): James Stivers, Kyle Seamon

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Enzymes, Plasmid, Recombinant Proteins, Research Reagent

Category(s): Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools, Technology Classifications > Research Tools > Proteins, Ligands & Receptors