Search Results - cloning+reagents

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A natural sg-tracrRNA for S. pyogenes transcriptional repression and cleavage

Unmet Need:CRISPR-Cas9 from Streptococcus pyogenes is the most commonly used due to Cas9’s ability to act as a singular protein and its high gene cutting activity (see Knott and Nakade). Despite the widespread use of CRISPR-Cas9, limitations remain in the efficiency with which precise genome editing occurs (see Banan). CRISPR-Cas9 creates a double-stranded...

Published: 3/13/2026 | Inventor(s): Joshua Modell

Keywords(s): Cloning Reagents, Discovery/Research Tools, Research Reagent

Category(s): Technology Classifications > Research Tools > Nucleic Acids

Transposon-Based Reporter Vector for High-Efficiency Regulatory DNA Sequence Screening

Value Proposition· Enhanced germline transmission - Tol2 transposon system provides superior integration efficiency compared to episomal DNA and alternative transposon systems· Streamlined cloning workflow - Gateway cloning system enables rapid insertion of test sequences without traditional restriction/ligation steps· Real-time...

Published: 3/12/2026 | Inventor(s): Elizabeth Grice, Shannon Fisher, Andrew McCallion

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Plasmid, Research Reagent

Category(s): Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools

Human LRRK2 cDNA

Studies have shown that mutations in the LRRK2 gene are the most common determinants of late-onset Parkinsons disease (PD) susceptibility. These cDNA vectors comprise wild type LRRK2 cDNA or mutated LRRK2 cDNA that can be expressed in cells to decipher the biochemical pathways and pathology of PD. Other advantages include:• Produced from human source...

Published: 3/12/2026 | Inventor(s): Ted Dawson

Keywords(s): Basic Research Biomarker, Cloning Reagents, CNS and Neurological Disorders, Discovery/Research Tools, Disease Indication, Nucleotide, Parkinson's Disease, Research Reagent

Category(s): Clinical and Disease Specializations, Technology Classifications > Research Tools > Nucleic Acids, Clinical and Disease Specializations > Neurology, Technology Classifications > Research Tools, Clinical and Disease Specializations > Neurology > Parkinson's Disease

Incorporation of Lysine Tags as a Universal System for Controlling Protein Expression

Value Proposition· Precise and Tunable Protein Translation Control: Poly(A) track length can be varied from 1-12 AAA codons, providing precise and adjustable control over protein output.· Minimal Complexity: This technology offers predictable, graded control of protein expression using only a short DNA sequence insert—no external inducers,...

Published: 3/12/2026 | Inventor(s): Dorothy Green, Sergej Djuranovic

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Nucleotide, Research Reagent

Category(s): Technology Classifications > Research Tools > Nucleic Acids, Technology Classifications > Research Tools

Identification of RNF43 Mutations in Pancreatic Cysts

Unmet Need· One third of diagnoses for surgical resection of pancreatic cysts are incorrect, leading to unnecessary surgery, medical risk, and high costs (Hammel, 2002). The current standard of care for determining pancreatic cyst type is based solely on symptom progression and imaging criteria, and histopathological analysis is only available...

Published: 3/12/2026 | Inventor(s): Bert Vogelstein, Kenneth Kinzler, Nickolas Papadopoulos, Jian Wu, Anirban Maitra, Ralph Hruban, Marco Dal Molin

Keywords(s): Cancers, Cloning Reagents, Discovery/Research Tools, Disease Indication, Nucleotide, Pancreatic Cancer, Research Reagent, Translational Research Biomarker

Category(s): Clinical and Disease Specializations, Clinical and Disease Specializations > Gastroenterology, Technology Classifications > Research Tools > Nucleic Acids, Clinical and Disease Specializations > Oncology, Technology Classifications > Research Tools, Clinical and Disease Specializations > Oncology > Pancreatic Cancer, Technology Classifications > Diagnostics > Biomarkers, Technology Classifications > Diagnostics > Diagnostic Biomarkers

Induced Pluripotent Stem Cell Lines (BC1 and C7) and Plasmids (pEB-C5, pEB-Tg)

The Material consists of two induced pluripotent stem (iPS) cell lines derived from human bone marrow and cord blood.and plasmids used to create these virus-free and integration-free human iPS cell lines.

Published: 3/12/2026 | Inventor(s): Linzhao Cheng

Keywords(s): Basic Research Biomarker, Cell Model, Cloning Reagents, Discovery/Research Tools, In Vitro Research Tool, Plasmid, Research Reagent, Stem Cells

Category(s): Technology Classifications > Research Tools > Cell Lines, Technology Classifications > Research Tools > Stem Cells, Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools

Single Vector Genome Editing with CRISPR/Cas and BacMam

Unmet NeedTargeted, site-specific genome editing is an important scientific tool with broad applications in basic science, the pharmaceutical industry, and translational medicine. It enables the addition of florescent or other types of gene tags for purification, visualization, or tracking. Genome editing can also introduce novel functions for endogenous...

Published: 3/13/2026 | Inventor(s): Chulan Kwon, Renjun Zhu, Amir Saberi

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Other Virus, Plasmid, Research Reagent, Translational Research Biomarker, Viruses

Category(s): Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools > Viruses

HIF-1(alpha)CA5: A Stable and Constitutively-Active Form of HIF-1(alpha)

Technical Overview: Animals and people are dependent on a continuous supply of O(2) for survival. Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that regulates oxygen homeostasis and plays key roles in development, physiology, and disease. Vascularization and vascular remodeling represent critical adaptive responses to tissue hypoxia...

Published: 3/12/2026 | Inventor(s): Gregg Semenza

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Nucleotide, Research Reagent

Category(s): Technology Classifications > Research Tools, Technology Classifications > Research Tools > Nucleic Acids

Use of PCR Fragments with Short Homology Arms for Genome Editing

Unmet NeedThe RNA-guided DNA endonuclease, Cas9, has emerged as a powerful new tool for genome engineering. Cas9 creates targeted ‘double-strand breaks’ (DSBs) in the genome. Knock-in of specific mutations (precision genome editing) requires ‘homology-directed repair’ (HDR) of the DSB by synthetic donor DNAs containing the desired edits, but HDR has...

Published: 3/13/2026 | Inventor(s): Geraldine Seydoux, Alexandre Paix

Keywords(s): Analytical Method, Cloning Reagents, Discovery/Research Tools, In Vitro Research Tool, Nucleotide, Research Reagent, Translational Research Biomarker

Category(s): Technology Classifications > Research Tools > Nucleic Acids

Plasmid Expressing P. Falciparum Aldolase Isolate 3D7

Unmet NeedApproximately 214 million malaria cases were recorded in 2015 with 438,000 people dying from this disease. The majority of these deaths result from a failure to diagnose and treat malaria before the onset of severe disease, at which point, even with proper treatment a 20% mortality rate may be expected. Microscopy is the current gold standard...

Published: 3/12/2026 | Inventor(s): David Sullivan

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Plasmid, Research Reagent

Category(s): Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools

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