Search Results - cloning+reagents

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Identification of RNF43 Mutations in Pancreatic Cysts

Unmet Need· One third of diagnoses for surgical resection of pancreatic cysts are incorrect, leading to unnecessary surgery, medical risk, and high costs (Hammel, 2002). The current standard of care for determining pancreatic cyst type is based solely on symptom progression and imaging criteria, and histopathological analysis is only available...

Published: 3/13/2025 | Inventor(s): Bert Vogelstein, Kenneth Kinzler, Nickolas Papadopoulos, Jian Wu, Anirban Maitra, Ralph Hruban, Marco Dal Molin

Keywords(s): Cancers, Cloning Reagents, Discovery/Research Tools, Disease Indication, Nucleotide, Pancreatic Cancer, Research Reagent, Translational Research Biomarker

Category(s): Clinical and Disease Specializations, Clinical and Disease Specializations > Gastroenterology, Technology Classifications > Research Tools > Nucleic Acids, Clinical and Disease Specializations > Oncology, Technology Classifications > Research Tools, Clinical and Disease Specializations > Oncology > Pancreatic Cancer, Technology Classifications > Diagnostics > Biomarkers, Technology Classifications > Diagnostics > Diagnostic Biomarkers

Induced Pluripotent Stem Cell Lines (BC1 and C7) and Plasmids (pEB-C5, pEB-Tg)

The Material consists of two induced pluripotent stem (iPS) cell lines derived from human bone marrow and cord blood.and plasmids used to create these virus-free and integration-free human iPS cell lines.

Published: 3/13/2025 | Inventor(s): Linzhao Cheng

Keywords(s): Basic Research Biomarker, Cell Model, Cloning Reagents, Discovery/Research Tools, In Vitro Research Tool, Plasmid, Research Reagent, Stem Cells

Category(s): Technology Classifications > Research Tools > Cell Lines, Technology Classifications > Research Tools > Stem Cells, Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools

Single Vector Genome Editing with CRISPR/Cas and BacMam

Unmet NeedTargeted, site-specific genome editing is an important scientific tool with broad applications in basic science, the pharmaceutical industry, and translational medicine. It enables the addition of florescent or other types of gene tags for purification, visualization, or tracking. Genome editing can also introduce novel functions for endogenous...

Published: 3/14/2025 | Inventor(s): Chulan Kwon, Renjun Zhu, Amir Saberi

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Other Virus, Plasmid, Research Reagent, Translational Research Biomarker, Viruses

Category(s): Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools > Viruses

HIF-1(alpha)CA5: A Stable and Constitutively-Active Form of HIF-1(alpha)

Technical Overview: Animals and people are dependent on a continuous supply of O(2) for survival. Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that regulates oxygen homeostasis and plays key roles in development, physiology, and disease. Vascularization and vascular remodeling represent critical adaptive responses to tissue hypoxia...

Published: 3/13/2025 | Inventor(s): Gregg Semenza

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Nucleotide, Research Reagent

Category(s): Technology Classifications > Research Tools, Technology Classifications > Research Tools > Nucleic Acids

Use of PCR Fragments with Short Homology Arms for Genome Editing

Unmet NeedThe RNA-guided DNA endonuclease, Cas9, has emerged as a powerful new tool for genome engineering. Cas9 creates targeted ‘double-strand breaks’ (DSBs) in the genome. Knock-in of specific mutations (precision genome editing) requires ‘homology-directed repair’ (HDR) of the DSB by synthetic donor DNAs containing the desired edits, but HDR has...

Published: 3/13/2025 | Inventor(s): Geraldine Seydoux, Alexandre Paix

Keywords(s): Analytical Method, Cloning Reagents, Discovery/Research Tools, In Vitro Research Tool, Nucleotide, Research Reagent, Translational Research Biomarker

Category(s): Technology Classifications > Research Tools > Nucleic Acids

Plasmid Expressing P. Falciparum Aldolase Isolate 3D7

Unmet NeedApproximately 214 million malaria cases were recorded in 2015 with 438,000 people dying from this disease. The majority of these deaths result from a failure to diagnose and treat malaria before the onset of severe disease, at which point, even with proper treatment a 20% mortality rate may be expected. Microscopy is the current gold standard...

Published: 3/13/2025 | Inventor(s): David Sullivan

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Plasmid, Research Reagent

Category(s): Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools

pAR-IRES-EGFP Plasmid

UNMET NEEDThe androgen receptor (AR) is a widely expressed ligand-activated transcription factor which mediates androgen signaling by binding to androgen response elements (AREs) in normal tissue and prostate cancer (PCa). Within tumors, the amount of AR plays a crucial role in determining cell growth, resistance to therapy and progression to fatal...

Published: 3/13/2025 | Inventor(s): John Isaacs

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Plasmid, Research Reagent

Category(s): Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools

Phosphatidylinositol 3-kinase (alpha) Expression Vectors

ABSTRACTResearchers at Johns Hopkins University have developed a set of plasmids that express recombinant P110a/niP85a heterodimer providing the means to purify large quantities of a fusion protein containing the full length phosphatidylinositol 3 kinase alpha (P110α) in complex with residues 322-600 of the regulatory subunit p85. The fusion protein...

Published: 3/13/2025 | Inventor(s): Bert Vogelstein, Sandra Gabelli

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Plasmid, Research Reagent

Category(s): Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools

shRNA knockdown vectors to inhibit expression of specific nuclear Lamin proteins and variants

Invention novelty:JHU researchers have designed and tested several several shRNA knockdown vectors that can inhibit specific isotypes of lamin proteins as well as variants that cause disease.Value Proposition:Many disease arise form aberration in proteins of the nuclear lamina. The researchers have designed and are testing several shRNA knockdown vectors...

Published: 3/13/2025 | Inventor(s): Xianrong Wong, Karen Reddy

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Plasmid, Research Reagent

Category(s): Technology Classifications > Research Tools > Vectors & Plasmids, Technology Classifications > Research Tools

DNA Constructs to Make Papillomavirus Pseudovirions for HPV Types

These are codon-optimized DNA constructs for the expression of different HPV types major capsid protein, L1 and minor capsid protein, L2, in the vector pVITRO.This Material is also available at Addgene: https://www.addgene.org/Richard_Roden/Related Publication:■ Vaccine. 2010 Jun 17; 28(28): 4478–4486. ■

Published: 3/13/2025 | Inventor(s): Richard Roden

Keywords(s): Basic Research Biomarker, Cloning Reagents, Discovery/Research Tools, Disease Indication, Human Papillomavirus, Human Papillomavirus (HPV), Infectious Diseases, Plasmid, Research Reagent, Viral Infections

Category(s): Clinical and Disease Specializations, Clinical and Disease Specializations > Infectious Diseases > Human Papillomavirus, Clinical and Disease Specializations > Infectious Diseases > Viral Infections, Technology Classifications > Research Tools > Vectors & Plasmids, Clinical and Disease Specializations > Infectious Diseases, Technology Classifications > Research Tools

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